We develop noninvasive molecular tools to control and monitor endogenous processes in intact cells and tissues, aiming to deepen the understanding of brain function in both health and disease. By combining therapeutic ultrasound, protein engineering, and pharmacology, we achieve noninvasive, spatiotemporal, and molecularly precise control and monitoring of endogenous molecules in their natural environment. Our work aspires to establish new paradigms in neuroscience and advance therapies for brain disorders.